Dendrobaena veneta earthworm - antiplatelet effect

The present work aimed to assess whether the different concentrations of the coelomic fluid of D. veneta may affect human platelets and blood coagulation.

List of used abbreviations:

PBS - Phosphate Buffered Saline

ASA – aspirin

Ekstrakt stęż. 25 – extract with a concentration of 25 µg/mL

Ekstrakt stęż. 50– extract with a concentration of 50 µg/mL

Ekstrakt stęż. 100 – extract with a concentration of 100 µg/mL

ASPI – ASPItest, aggregation in response to 0.5 mM arachidonic acid (aggregation in response to 0.5 mM arachidonic acid)

ADP – ADPtest, platelet aggregation in response to 6.5 µM adenosine-5′-diphosphate

TRAP – TRAPtest, aggregation in response to 32 µM thrombin receptor activating peptide 6 (aggregation in response to 32 µM thrombin receptor activating peptide 6)

PT – prothrombin time

U, UA – unit used in ASPItest, ADPtest, TRAPtest

INR – International Normalized Ratio, used to monitor the effectiveness of anticoagulants

PR - protrombin ratio (%)

Equipment used for ASPI, ADP, TRAP tests: Multiplate® Platelet Function Testing 5.0 Analyzer (Roche, Rotkreuz, Switzerland