SDAV infection and role of VCP in primary murine neurons

This study was designed to understand and investigate the involvement of valosin-containing protein (VCP), an ATPase required for ubiquitin-mediated proteasomal degradation of proteins, in neuronal infection. Sialodacryoadenitis virus (SDAV), a member of the genus Betacoronavirus, was used for the first time in such a study to gain a better understanding of SDAV pathogenesis. We targeted the valosin-containing protein because of its critical role in cell metabolism and viral replication.

Data deposited in the repository was saved in .xlsx format for numerical data, .jpg, and .tiff format for microscopy images. Uploaded folders contain raw data obtained via Revvity Operetta CLS and ThermoFisher Array Scan XTI high content throughput microscopes (protein and virus count, viral titer, cell morphology), Olympus FV10i confocal microscope (cell morphology), BioRad Chemidoc (Western Blot analysis), BioTek Synergy H1 plate reader (XTT test), and JuliBr live imaging system (observation on cells in time).