Florescent in situ hybridization with rDNA probes to the chromosomes of annual Brachypodium species.

The aim of the study was to draw a complex picture of rRNA gene evolutionary trajectories in two Brachypodium hybridum lineages of distinct evolutionary ages and cross directions. We aimed to address the following questions: (i) are the evolutionary trajectories of 35S and 5S rDNA homoeologues similar or not?; (ii) is there a relationship between the direction of the cross and rDNA homoeologues loss in B. hybridum?; (iii) do the rRNA genes in the studied B. hybridum lineages of different evolutionary ages follow the same evolutionary pathways?

Description of the database: Microphotographs (TIF files) of mitotic metaphase chromosomes after fluorescence in situ hybridisation (FISH) with 25S rDNA and 5S rDNA as probes. All microphotographs were acquired using a Zeiss AxioImager.Z.2 fluorescent microscope equipped with an AxioCamHMr camera (Zeiss, Oberkochen, Germany). The microphotographs were processed uniformly using ZEN 2.3 Pro (Zeiss). 

Number of TIF files: 12.