Myopathy-linked mutations in TPM2 and their influence on troponin-mediated regulation of actomyosin contractility

The files are a collection of raw experimental data that were used to prepare figures which are to be published in the article under the title: "Myopathy-linked mutations in TPM2 and their influence on troponin-mediated regulation of actomyosin contractility"

The ATPase data sets file contains the collection of raw data obtained from the spectrophotometer during myosin-S1 ATPase analyzes and the analysis of these raw data sets. Temperature dependence ATPase data sets file contains the collection of raw data obtained from the spectrophotometer during myosin-S1 ATPase analyzes at different temperature and the analysis of these raw data sets.

Tpm interactions with Tn (ELISA) data sets file contains the collection of raw data obtained from the spectrophotometer during ELISA analyzes and the analysis of these raw data sets.

The Invitro motility assay data sets file contains the collection of actomyosin trajectories velocity data obtained from the analysis of the captured videos by the automatic program and the analysis of these raw data sets.

Raw data collected from separate experiments shown as troponin-I per actin densitometric ratio. The averaged data and errors calculated from the independent experiments. Raw data collected from separate experiments shown as troponin-T per actin densitometric ratio. The averaged data and errors calculated from the independent experiments.

Pictures of SDS gel electrophoresis of F-actin pellet containing troponin with Tpm2.2 variants were used for densitometric analysis of protein bands.