Wpływ transformacji genem taglu na wzrost i rozwój transgenicznej truskawki w kulturach in vitro

The aim of the presented work was to study the effects of changes of endogenous indole-3-acetic acid (lAA) metabolizm on in vitro shoot proliferation and rhizogenesis of transgenic strawberry shoots carrying maize lAA-glucose synthase gene (iaglu). Four /ag/u-transformed strawberry clones and non- transformed ‘Raster’ shoots served as a plant material for the study. The analysis of free and conjugated lAA level in leaves of transgenic and control strawberry plants showed that /og/u-containing strawberry clones had significantly higher level of ester conjugated lAA, but the level of free hormone was only slightly decreased or comparable to the control plants, mg/u-transformed clones had significantly higher proliferation rate and formed more roots than the control shoots. One of the /og/u-transformed clones had significantly shorter and other two - longer roots than the control plantlets.