Artificially engineered specific nucleases - a breakthrough in RE research - Rewiev

artykuł promocyjny

During the last few decades, several hundred type II restriction enzymes have been isolated and characterized from variousbacterial strains, and yet, many specificities are still unavailable.There is an increasing demand for a wider selection of restriction enzymes with varying recognition sequences, which hasstimulated efforts to produce artificial restriction enzymes. Therapidly expanding field of protein engineering couples studies ofprotein structure and function with molecular evolution basedon random mutagenesis and high throughput screening to create enzymes having desired properties.