Metoda różnicowego namnażania sposób poszukiwania genów ulegających specyficznej ekspresji

Identification of differentially expressed genes is one of the major challenges in molecular biology. Several techniques allow the cloning of such sequences. How'ever, methods such as differential hybridization are time-consuming and require large amounts of mRNA. Recently a new approach has been successfully developed: differential display by polymerase chain reaction (DD PCR).This technique has been proven to be highly effective in identifying sequencesthat are differentially expressed in various cell types.