Transgene expression in plant cell

Transgene incorporation in plant genome does not mean that the gene willbe active and will show expression at the required level. It is often the case thattransgenic plants do not exhibit the activity of the gene introduced. Geneexpression is known to be dependent on many factors such as: chimeric genestructure, proper and stable integration to the genome and proper transcription and translation. The knowledge of transgene regulation is essential for theunderstanding of the transcription and translation mechanisms. Each plazmidcomponent is vital for later transgene expression. This refers to the size ofT-DNAintroduced, location of particular elements, leader sequences length, AUG sequences, introns presence, optimum coding gene synthesis, removal of RNA instability signals and many other possible modifications.