Wytwarzanie etanolu z laktozy przez komórki drożdży unieruchomione w kulkach agaru porowatego

Microinjection is one of the most successfully used methods to produce transgenic farmanimals. But the effectiveness of transgenesis with the use of microinjection, specially in cattleis still low. Many steps of the Iransgenesis has been found to influence its effectiveness; DNApurity, the site of its injection, the culture system. I'here are no reports on the influence of aDNA vector concentration influence on the developmental ability of transformed bovine eggs tothe blastocyst stage in the in vitro culture. In presented experiments we investigated the influenceof different DNA concentrations on the developmental rate of microinjected immature bovineoocytes, zygotes and 2-cell embryos to the blastocyst stage.

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