Continuous sterilization of medium for bacitracin biosynthesis

The aim of the paper was to estimate the applicability of continuous sterilization to theimprovement of bacitracin production technology. Preliminary investigations were carried outand the effects of application of thermal continuous sterilization of substrates were estimated.The quality of sterilized substrates was assessed in fermentation tests. Bacitracin biosynthesiswas carried out on a substrate sterilized by both batch and continuous method. In laboratoryinvestigations a substrate sterilized by the continuous method enabled to increase antibioticproduction by 30% on the average as compared to the yield obtained using a batch sterilizedsubstrate. On an industrial scale, for the same inoculation material the production was evenhigher by 80%.