Influence of reaction conditions on yield of enzymatic synthesis galactooligosacharides by Penicillium canescens B-galactosldase

The aim of the work was to examine the ability of Penicillium canescens P-galactosidase to synthesize galactooligosacharides in one or two-phase medium (organic solvents: water). The yield of this process depends mainly on: lactose concentration in water phase, time of reaction, kind of organic solvents and their participation in two-phase medium. The application of ultrafiltration for separation of saccharides from the reaction medium stabilized the yield of galactooligosacharides synthesis.