Application of ultra-high dose rate (FLASH) proton beam for the 3D cancer cell model : a proof of concept

Ultra-high dose rate (FLASH) proton radiotherapy is a promising treatment method for cancer patients. In our research, we want to compare the FLASH method with a conventional radiation method to show what effect they have on the biochemical structure of the tumour (3D model - spheroids) and the secretion of EVs and their cargo. The use of a modern method of creating spheroids will enable us to create conditions that are more able to mimic the tumour microenvironment.